Throughout the film performances are being put on by various characters to ensure the success of operation kino.  Due to the German speaking bastards being caught, these Americans have to portray Italians in order to get into the premiere of the film.  This scene built up all the tension from before because the cover that was in place was once very believable and is now at the hands of unqualified Americans.  This meme pokes fun at not only the circumstances that the bastards are in but the fact that Americans are really bad at speaking other languages without using the American alphabet and accent.  You can tell with how Col Landa is mocking their accents in this scene when he makes them repeat their name several times egging them on to put everything they have into it. Col Landa himself shows that he speaks Italian which shows the audience not only how clever he is as a man but how screwed the bastards are and the eventual death that occurs with Bridget.